Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Dual-process brain mitochondria isolation preserves function and clarifies protein composition

doi: 10.1073/pnas.2019046118

Figure Lengend Snippet: Citation information for cellular localization of proteins previously reported as localizing in mitochondria but not observed in ultrapurified mitochondria here

Article Snippet: Antibodies included Anti-Ca v 1.2 (Alomone Labs, AGP-001), Gapdh (Santa Cruz, sc-365062), Sdha (Cell Signaling, 11998), Tom20 (Cell Signaling, 42406), Facl4 (ThermoFisher, PA5-27137), Tom70 (Santa Cruz Biotechnology, sc-390545, a gift from Stefan Strack, University of Iowa, Iowa City, IA), Pdh E1α (Cell Signaling, 3205), Vdac (Cell Signaling, 4661), Serca2 (Cell Signaling, 4388), Erlin2 (Cell Signaling, 2959), Psd95 (Cell Signaling, 2507), β-actin (Santa Cruz, sc-47778), Eaat1 (Alomone, AGC-021), Glut3 (Millipore, AB1344), Mct1 (Alomone, AMT-011), Asic1 (biorbyt, orb13257), K v 1.3 (Alomone, APC-002), Ryr1 (Cell Signaling, 8153), mitoK (Sigma, HPA010980), Slc25a12 (Alomone, ANT-112), Slc25a22 (Proteintech, 25402-1-AP), Mcu (Sigma, HPA016480, a gift from Yuriy Usachev, University of Iowa), Mpc1 (Cell Signaling, 14462S), and Mpc2 (Cell Signaling, 46141S).

Techniques: Clinical Proteomics, Membrane, Fractionation

Journal: Microcirculation (New York, N.Y. : 1994)

Article Title: Aging alters spontaneous and neurotransmitter-mediated Ca 2+ signaling in smooth muscle cells of mouse mesenteric arteries

doi: 10.1111/micc.12607

Figure Lengend Snippet: Transcript levels for ryanodine receptor isoforms in mesenteric artery smooth muscle cells from young and old mice. Data are from 22 with permission.

Article Snippet: Thus prepared, slides were incubated 60 min in primary antibody [Alamone Labs, 1:250] for RyR1 (Cat. #ARR-001), RyR2 (Cat. #ARR-002), or RyR3 (Cat. #ARR-003).

Techniques:

Journal: Microcirculation (New York, N.Y. : 1994)

Article Title: Aging alters spontaneous and neurotransmitter-mediated Ca 2+ signaling in smooth muscle cells of mouse mesenteric arteries

doi: 10.1111/micc.12607

Figure Lengend Snippet: Representative immunofluorescence images depicting green fluorescence for RyR1 (top rows), RyR2 (center rows) and RyR3 (bottom rows) in 3 separate SMCs from MAs of (A) young and (B) old mice, (C) SMCs incubated with respective blocking peptides, and (D) SMC with primary omitted. ToPro nuclear stain (blue) is included in all images. Scale bars = 20 μm and apply to all panels.

Article Snippet: Thus prepared, slides were incubated 60 min in primary antibody [Alamone Labs, 1:250] for RyR1 (Cat. #ARR-001), RyR2 (Cat. #ARR-002), or RyR3 (Cat. #ARR-003).

Techniques: Immunofluorescence, Fluorescence, Incubation, Blocking Assay, Staining

Journal: Microcirculation (New York, N.Y. : 1994)

Article Title: Aging alters spontaneous and neurotransmitter-mediated Ca 2+ signaling in smooth muscle cells of mouse mesenteric arteries

doi: 10.1111/micc.12607

Figure Lengend Snippet: Transcript levels for ryanodine receptor isoforms in mesenteric artery smooth muscle cells from young and old mice. Data are from 22 with permission.

Article Snippet: Thus prepared, slides were incubated 60 min in primary antibody [Alamone Labs, 1:250] for RyR1 (Cat. #ARR-001), RyR2 (Cat. #ARR-002), or RyR3 (Cat. #ARR-003).

Techniques:

Journal: Microcirculation (New York, N.Y. : 1994)

Article Title: Aging alters spontaneous and neurotransmitter-mediated Ca 2+ signaling in smooth muscle cells of mouse mesenteric arteries

doi: 10.1111/micc.12607

Figure Lengend Snippet: Representative immunofluorescence images depicting green fluorescence for RyR1 (top rows), RyR2 (center rows) and RyR3 (bottom rows) in 3 separate SMCs from MAs of (A) young and (B) old mice, (C) SMCs incubated with respective blocking peptides, and (D) SMC with primary omitted. ToPro nuclear stain (blue) is included in all images. Scale bars = 20 μm and apply to all panels.

Article Snippet: Thus prepared, slides were incubated 60 min in primary antibody [Alamone Labs, 1:250] for RyR1 (Cat. #ARR-001), RyR2 (Cat. #ARR-002), or RyR3 (Cat. #ARR-003).

Techniques: Immunofluorescence, Fluorescence, Incubation, Blocking Assay, Staining